Extended Data Fig. 4: 10x VISIUM Spatial sequencing of the mouse globe and optic nerve.
From: Compartmentalized ocular lymphatic system mediates eye–brain immunity
Mouse eyes were processed using traditional FFPE-based histology preparation. Samples were processed using the 10x VISIUM platform and analyzed using Seurat. a, UMAP of different spatial sequencing blocks with categorization into overall tissue level structure. b, Projection of tissue-type clusters onto the globe and surrounding structures. c, Image of H&E staining and respective cell type gene signature projected onto spatial sequencing data (yellow arrows denote where the gene signature appears for given signature).
