Extended Data Fig. 6: Cryo-EM processing and reconstruction of β2AR-GsGTP(Merged).
From: Time-resolved cryo-EM of G-protein activation by a GPCR
a, Flow chart outlining the cryo-EM processing of β2AR-GsGTP(Merged) complex using cryoSPARC29,65. Local refinement reconstructions are shown with a Gaussian filtered map outline to show micelle and AHD densities. The percent contribution of particles from each dataset to each local refinement is shown next to each reconstruction (orange, 5 sec.; blue, 10 sec.; green, 17 sec.). b, Local resolution of projections used in final cryo-EM reconstructions. See Supplementary Fig. 1 for associated 3DFSC94 curves, directional orientation, power spectra, and angular distribution maps; and see Supplementary Table 2 for a table of sphericity scores.
