Extended Data Fig. 1: Yeast exoproteome library composition and diversity and bacterial strain collection composition and diversity.
From: A host–microbiota interactome reveals extensive transkingdom connectivity
a, Extracellular protein sequences are curated and cloned into a standardized backbone featuring a C-terminal epitope tag. Proper display is confirmed via epitope tag staining, as well as binding by confirmation-specific antibodies or endogenous ligands for a subset of proteins. b, Schematic of expression construct used in the yeast display library. c, Proportion of the human exoproteome represented in the yeast display library. d, Each protein is represented by multiple barcodes, with a median of 20 barcodes per protein. Boxplot shows median, IQR, and whiskers extend to 1.5x IQR for n = 3,406 epitopes from 3,336 proteins in the library. e, Tissue expression (defined as Normalized Expression (NX) > 10 in the Human Protein Atlas) of proteins in the library, grouped by barrier, immune, and sterile tissues. f, Percentage of proteins in the library belonging to highly represented protein families. g, Number of strains from indicated genera, showing all genera with 9 or more strains. h, Number of strains from indicated species, showing all species with 5 or more strains. i, Number of strains from different body sites, showing all body sites with 5 or more strains.
