Extended Data Fig. 3: Calibration of optogenetic stimulus strength.
From: Motor neurons generate pose-targeted movements via proprioceptive sculpting
(a) Example trace of the membrane potential (vertical scale bar = 10 mV) of the CvN7 motor neuron in response to a 300 ms flash of 625 nm, 0.04 mW/mm2 light. Underneath the voltage trace is a raster indicating the timing of action potentials from multiple trials. (b) An action field plotting the head movements resulting from stimulating CvN7 with the same 0.04 mW/mm2 light pulse. (c) A plot of the ‘rotational difference’ between the action field in b and the equivalent data that occurs during the no-stimulus control condition – see Fig. 2a–c for more detail. (d) The neural response of CvN7 to a 0.09 mW/mm2 stimulus. (e) The CvN7 action field resulting from a 0.09 mW/mm2 stimulus. (f) A plot of the ‘rotational difference’ between the action field in e and the equivalent data that occurs during the no-stimulus control condition. (g) The neural response of CvN7 to a 0.37 mW/mm2 stimulus. (h) The CvN7 action field resulting from a 0.37 mW/mm2 stimulus. (i) A plot of the ‘rotational difference’ between the action field in h and the equivalent data that occurs during the no-stimulus control condition. (j) The neural response of CvN7 to a 1.43 mW/mm2 stimulus. (k) The CvN7 action field resulting from a 1.43 mW/mm2 stimulus. (l) A plot of the ‘rotational difference’ between the action field in k and the equivalent data that occurs during the no-stimulus control condition. Genotype for all panels: SS00754 x w +, norpA, 20XUAS-CsChrimson-mVenus (attP18).
