Extended Data Fig. 4: Effect of induced-proximity dependency, tag location and linker size on effector activity.
From: Proteome-scale discovery of protein degradation and stabilization effectors
(a) The EGFP-ABI1 293T reporter cell line was transfected with indicated effectors fused to vhhGFP or 3xFLAG. EGFP fluorescence was measured by flow cytometry and normalized to cells transfected with an unrelated construct (no effector). D, Degrader hit; S, Stabilizer hit. (b) The EGFP-ABI1 293T reporter cell line was transfected with indicated effectors fused in their C terminus or N terminus. EGFP fluorescence was measured and normalized as described in (a). (d) Indicated C-terminal vhGFP fusions cloned either with the original or a minimal linker were transfected in the EGFP-ABI1 293T reporter cell line. EGFP fluorescence was measured and normalized as described in (a). Of the two independent experiments performed, results from one representative experiment are shown.
