Extended Data Fig. 6: UBE2B mutations and truncations.
From: Proteome-scale discovery of protein degradation and stabilization effectors
(a) Western blot analysis of 293T cells transfected with wild-type UBE2B-vhhGFP or the catalytically inactive mutant C88A. (b) Expression of UBE2B mutants deficient in E3 binding transfected with the indicated vhhGFP fusions into 293T cells. RQRR, S25R/V39Q/N65R/T99R quadruple mutant. (c) Structure of UBE2B (PDB 2YB6). Fragments 1–18 (light orange), 124–134 (black), and 134–152 (grey) are indicated. (d) Western blot analysis of 293T transfected with wild-type or truncated UBE2B-vhhGFP. (e) Effect of UBE2B truncation on proximity-dependent degradation. The EGFP-ABI1 293T reporter cell line was transfected with indicated vhhGFP fusions and reporter stability was measured by flow cytometry. Of the two independent experiments performed for (a), (b) and (d), results from one representative experiment are shown.
