Extended Data Fig. 10: Loss of EGFP signal is due to EGFP ubiquitin-mediated proteolysis.
From: Proteome-scale discovery of protein degradation and stabilization effectors
293T cells were co-transfected with indicated effectors fused to vhhGFP and EGFP or EGFP13R fused to either ABI1 or to one of two lysine-less substrates, human p14ARF and oncoprotein E7 from human papillomavirus 58 (HPV58). EGFP13R is a GFP mutant where thirteen surface lysines are mutated to arginines. Of note, the vhhGFP epitope is not mutated in EGFP13R. EGFP fluorescence was measured 48 h after transfection by flow cytometry.
