Extended Data Fig. 1: Establishing the induced proximity assay.
From: Proteome-scale discovery of protein degradation and stabilization effectors
(a) Representative flow cytometry plots of the EGFP-ABI1-IRES-TagBFP dual-reporter 293T stable line transduced with C-terminal PYL1 fusions. SPOPΔMATH is lacking its native substrate-binding domain (residues 28–166). Cells were treated with either 0.5% DMSO or 100 µM abscisic acid (ABA) for 24 h. (b) Frequency of high EGFP population as a function of ABA concentration and treatment time in the reporter cell line transduced with the C-terminal SPOPΔMATH-PYL1 fusion. (c) Representative flow cytometry plots of the EGFP-ABI1-IRES-TagBFP dual-reporter 293T stable line transduced with C-terminal vhhGFP fusions of Nanoluc, CRBN, full-length SPOP, or SPOPΔMATH.
