Fig. 1: Acute heat reduces food intake and selectively activates α-tanycytes.
From: A brainstem–hypothalamus neuronal circuit reduces feeding upon heat exposure
a, Schematic of the experimental paradigm to link acute thermal challenge (1 h) to reduced food intake in wild-type male and female mice. b, Food intake in male and female mice during a 24-h period after exposure to 40 °C (1 h) versus continuity at 25 °C (n = 8 per sex). c, Core body temperature during (pink shaded region) and after thermal challenge. Data are presented as a facet-wrap plot with measurements taken at 15-min intervals (n = 8 male mice). d, Acute heat-induced cFOS expression in the PBN (arrows). scp, superior cerebellar peduncle. Scale bar, 120 μm. e, cFOS immunoreactivity in vimentin+ α-tanycytes (at −2.30 mm relative to bregma) at the indicated temperatures (n = 3 male mice per group; data for female mice are in Extended Data Fig. 1e). Scale bars, 4 µm. f, The number of cFOS+ α-tanycytes per 50 µm of ventricular surface in control versus heat-exposed mice of both sexes. Data are mean ± s.e.m., with circles (b,f) denoting individual data points. b,c,f, Detailed statistics are provided in Methods. *P < 0.05, **P < 0.01, ***P < 0.001.
