Extended Data Fig. 5: Chemogenetic induction of PBN neurons activates tanycytes.
From: A brainstem–hypothalamus neuronal circuit reduces feeding upon heat exposure
a. Confocal images along the wall of the 3rd ventricle (−1.94 mm, relative to bregma) showed cFOS activation (in grey) both in vimentin+ α-tanycytes (in green) and neurons following an i.p. injection of either saline or CNO (5 mg/kg; 1.5 h) in Slc17a6-IRES2-FlpO-D mice in which AAV2-Syn1-FRT-hM3D(Gq)-mCherry particles were stereotaxically delivered to the PBN 21 days earlier. Scale bar = 15 µm. a1. The number of cFOS+ α-tanycytes (−2.30 mm, relative to bregma) was expressed along 50 μm ventricular wall segments. Data in bar graphs are means ± s.e.m.; for saline (grey, n = 5) and CNO-injected mice (5 mg/kg in red, n = 6). Open circles correspond to individual data points; t = 4.732; p = 0.001; Student’s t-test.
