Extended Data Fig. 1: The assembly of the Pol II Rat1-PTTC1 and Pol II Rat1-PTTC2.
From: Structural basis of exoribonuclease-mediated mRNA transcription termination
a-b, Elution peaks and SDS-PAGE results of Pol II Rat1-PTTC1 from a size-exclusion chromatography. c-d, Elution peaks and SDS-PAGE result of Pol II Rat1-PTTC2 from a size-exclusion chromatography. Asterisks indicate degradation products of Rpb1. e, The Pol II release result shows the Rat1-Rai1 complex releases Pol II from the elongation complex. Three biological repeats were done, see Supplementary Fig. 1. f, The RNA cleavage result shows the Rat1-Rai1 complex has exoribonuclease activity. Three biological repeats were done, see Supplementary Fig. 1.
