Extended Data Fig. 2: Cryo-EM data processing of Pol II Rat1-PTTC1.
From: Structural basis of exoribonuclease-mediated mRNA transcription termination
a, The flowchart of data processing for Pol II Rat1-PTTC1. b, Representative cryo-EM micrograph. c, Representative 2D class averages. d-f, Gold-standard FSC curves of map 1a, map 1b, and map 1c. g, The 3D FSC plot. The dotted line represents 0.143 cutoff of the global FSC curve. h, Nominal 2.7 Å-resolution cryo-EM reconstruction of map 2a filtered by local resolution. i-j, Map classified from Pol II Rat1-PTTC1. Map 1a represents the Pol II Rat1-PTTC1. Map 1c represents Pol II Spt4-Spt5 bound TEC. The Rat1-Rai1 map is colored green, and the Spt4-Spt5 map is colored blue.
