Extended Data Fig. 9: Phenotype and function of anti-Lewis Y CAR T cells following CRISPR/Cas9-mediated deletion of FOXO1.
From: FOXO1 enhances CAR T cell stemness, metabolic fitness and efficacy
Anti-Lewis Y CAR T cells deficient for FOXO1 were generated via CRISPR/Cas9 targeting. The expression of CD62L and CD27 on CD8+CAR+ T cells (a-b) and CD4+CAR+ T cells (c-d) were determined over 28 days in culture with IL-2. a, c, Representative flow cytometry plots of 3 independent donors. b, d, Numbers of total (left) or CD27+CD62L+ (right) CD8 + CAR T cells. Data represents the mean ± SD of technical triplicates from a representative experiment of n = 3 donors. Statistical significances determined by unpaired two-sided t test. e, CAR T cells were cocultured overnight at a 1:1 ratio with OVCAR-3 or MCF7 tumor cells for 16 h and the production of IFNγ or TNF determined by CBA. Data represents the mean ± SD of technical triplicates from a representative experiment of n = 3 donors. Statistical significance determined by unpaired two-sided t test, (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided in the Source Data file.
