Extended Data Fig. 7: EP₂/EP₄ double deficiency rescues development of early and terminally differentiated effector CD8⁺ T cells within tumour tissue.

a-c, WT, Ptger2^−/−Ptger4^fl/fl or Cd4^crePtger2^−/−Ptger4^fl/fl mice were injected s.c. with 2×10⁶ control or Ptgs1/Ptgs2^−/− BRAF^V600E cells and CD44⁺ CD8⁺ TILs were analysed 11 days later by flow cytometry. a, Representative plots showing the frequency of TCF1⁺ stem-like and TIM-3⁺ effector CD8⁺ TIL populations. b, Quantification of TCF1⁺CD8⁺ TILs (Ptgs1/Ptgs2^−/− into WT, n = 9; Control into WT, n = 11; Control into Ptger2^−/−Ptger4^fl/fl, n = 7; Control into Cd4^crePtger2^−/−Ptger4^fl/fl, n = 10). c, Quantification of TIM-3⁺CD8⁺ TILs (Ptgs1/Ptgs2^−/− into WT, n = 8; Control into WT, n = 10; Control into Ptger2^−/−Ptger4^fl/fl, n = 7; Control into Cd4^crePtger2^−/−Ptger4^fl/fl, n = 10). Data in b,c are pooled from three independent experiments and depicted as mean ± s.e.m. Plots in a show data for one tumour sample representative for at least n = 7 samples from three independent experiments. P values are from one-way ANOVA with Tukey’s multiple-comparison test. P ≥ 0.05, not significant (NS).

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