Fig. 4: EP2/EP4-deficient tumour antigen-specific CD8+ T cells expand in PGE2-producing tumours and mediate tumour immune control.
From: PGE2 limits effector expansion of tumour-infiltrating stem-like CD8+ T cells
a, Experimental design for b–f. b, Flow cytometric plots of CD8+ T cells from tdLNs and tumours from the indicated days. c,d, Numbers of expanded OT-I CD8+ T cells in tdLNs (c) and tumours (d) at indicated time points (n = 6). e,f, Analysis of CD44 and CXCR6 expression in Cd4crePtger2−/−Ptger4fl/fl OT-I cells. e, Flow cytometry plots. f, Subset frequencies (n = 6). g–j, Effect of CD122/CD132 blockade on OT-I T cell expansion in tumours. g–j, Effect of anti-CD122 and anti-CD132 (anti-CD122/CD132) treatment on OT-I TIL expansion in WT mice with control or Ptgs1/Ptgs2−/− BRAFV600E-OVA tumours or with MC38-OVA tumours, analysed 11 days after tumour transplantation. g,h, Flow cytometry plots (g) and OT-I TIL numbers (h) in BRAFV600E-OVA tumours (n = 6). i,j, Flow cytometry plots (i) and OT-I TIL numbers (j) in MC38-OVA tumours (n = 10). k, Experimental design for l and m with MC38-OVA tumours. l, Flow cytometry plot (left) and quantification (right) of OT-I TILs at day 10 (n = 6). m, Flow cytometry plots showing the population size of TIM-3+CXCR6+ cells among control and Cd122−/− Cd4crePtger2−/−Ptger4fl/fl OT-I TILs. n, WT mice received 1 × 103 naive OT-I T cells or 1 × 103 naive Cd4crePtger2−/−Ptger4fl/fl OT-I T cells intravenously (i.v.) and were transplanted s.c. with 2 × 106 MC38-OVA cells before analysis of tumour growth over time (n = 10). Asterisk indicates that termination criteria were reached. Data in c, d, f, h, j, l and n are pooled from two (c,d,h,l) or three (f,j,n) independent experiments and depicted as box plots extending from the 25th to 75th percentiles with the median as the centre and the whiskers corresponding to minimum and maximum values (c,d,h,j,l) or shown as the mean ± s.e.m. (f,n). Plots in b, e, g, i, l and m show data for 1 sample representative of n = 6 samples analysed in 2 (b,g,l,m) or 3 (e,i) independent experiments. P values are from paired t-tests (l), one-way ANOVA with Tukey’s multiple-comparison test (c,d) or Dunnett’s multiple-comparison test (h,j), or two-way ANOVA with Bonferroni’s correction for multiple testing (n).
