Extended Data Fig. 9: MLKL binding requires the displacement of the RIPK3 αC helix.
From: Necroptosis blockade prevents lung injury in severe influenza
a,b, Structures of monomeric (a) and MLKL-bound (b) mRIPK3. N-terminal lobe is depicted in gold and the C-lobe in light green. The αC helix is shown in light blue, and the beginning of the activation loop, including the DFG motif, in salmon. The side chains of key active site residues are shown as sticks and labeled. c. Overlaying the monomeric and MLKL-bound conformations of mRIPK3 shows that the αC helix swivels (blue arrow) outwards in the MLKL-bound conformation, with the E61 residue now facing away from the active site. The dashed red lines indicate axes of the αC helices in inactive and active conformations.
