Extended Data Fig. 7: Cryo-EM structures of FLVCR1 determined in 1mM ethanolamine.
From: Structural basis of lipid head group entry to the Kennedy pathway by FLVCR1
a-b, Cryo-EM density maps of endogenous choline-bound FLVCR1 (a) and ethanolamine-bound FLVCR1 (b) states, determined from particles imaged in the presence of 1 mM ethanolamine. c, Superposition of ethanolamine-bound (gold) and choline-bound (blue) states. d, Substrate-binding site in endogenous choline-bound FLVCR1 state, determined from particles imaged in the presence of 1 mM ethanolamine. Residues and modelled substrates are shown as sticks. Density is shown as a grey isosurface and contoured at 3.0 σ. e-f, Coordination of ethanolamine in the substrate-binding site in ethanolamine-bound state (e) and choline in the substrate-binding site in the choline-bound state (f). Polar interactions are shown as dashed lines and distances as solid lines.
