Extended Data Fig. 1: Regulation of FLVCR1-mediated choline uptake.
From: Structural basis of lipid head group entry to the Kennedy pathway by FLVCR1
a, Background subtracted uptake of 20 nM [methyl-3H]choline by vesicles containing FLVCR1 after 30 min in buffer at pH 4.5-10.5. Background was measured by uptake of 20 nM [methyl-3H]choline into the protein-free control liposomes. n = 3 technically independent samples. b, Background subtracted uptake of 20 nM [methyl-3H]choline by FLVCR1 proteoliposome after 30 min in buffer in the presence or absence of Na+, K+, Ca2+, or Mg2+. Background was measured by uptake of 20 nM [methyl-3H]choline into the protein-free control liposomes. n = 3 technically independent samples.
