Extended Data Fig. 2: Validation of cryo-EM structures of FLVCR1.
From: Structural basis of lipid head group entry to the Kennedy pathway by FLVCR1
a-c Representative cryo-EM images and two-dimensional class averages of human FLVCR1 in 1 mM choline (a), 1 mM ethanolamine (b) or without added substrate (c). Number of collected cryo-EM images are shown. d-h, Plots showing Fourier shell correlations between two independent half-maps (black) and between density-modified map and refined atomic model (red) for choline-bound FLVCR1 (d), ethanolamine-bound FLVCR1 (e), endogenous choline-bound FLVCR1 obtained from FLVCR1 incubated with 1 mM ethanolamine (f), endogenous choline-bound FLVCR1 obtained from FLVCR1 without exogenous ligand incubation (g), and endogenous ligand-bound FLVCR1 obtained from FLVCR1 without exogenous ligand incubation (h). Dashed lines are indicated at FSC = 0.5 and FSC = 0.143. i-m, Local resolution plots of choline-bound FLVCR1 (i), ethanolamine-bound FLVCR1 (j), endogenous-choline bound FLVCR1 obtained from FLVCR1 incubated with 1 mM ethanolamine (k), endogenous choline-bound FLVCR1 obtained from FLVCR1 without exogenous substrate incubation (l), and endogenous ligand-bound FLVCR1 obtained from FLVCR1 without exogenous ligand incubation (m).
