Fig. 5: COV-2130 and 2130-1-0114-112 escape mapping using DMS.
From: Computationally restoring the potency of a clinical antibody against Omicron
a,b, Comparison between IC50 values measured using DMS for COV-2130 and 2130-1-0114-112 antibodies in BA.1 (a) and BA.2 (b) backgrounds, with key mutations highlighted. Arbitrary units in both plots are on the same scale. Interactive plots that display each mutation can be found at https://dms-vep.org/SARS-CoV-2_Omicron_BA.1_spike_DMS_COV2-2130/compare_IC50s.html for the BA.1 background and at https://dms-vep.org/SARS-CoV-2_Omicron_BA.2_spike_DMS_COV2-2130/compare_IC50s.html for the BA.2 background. c,d, Heatmaps of mutation escape scores at key sites for each antibody in BA.1 (c) and BA.2 (d) backgrounds. Escape scores were calculated relative to the wild-type amino acid in the same virus background. X marks wild-type amino acid in the relevant background. Amino acids not present in the DMS libraries lack squares; grey squares are mutations that strongly impair spike-mediated infection. Mutations identified in a,b are shown with a heavy line surrounding the corresponding box in c,d. Interactive heatmaps for full spike can be found for the BA.1 background at https://dms-vep.org/SARS-CoV-2_Omicron_BA.1_spike_DMS_COV2-2130/COV2-2130_vs_2130-1-0114-112_escape.html and https://dms-vep.org/SARS-CoV-2_Omicron_BA.2_spike_DMS_COV2-2130/COV2-2130_vs_2130-1-0114-112_escape.html for the BA.2 background.
