Extended Data Fig. 1: Optimized, single-concentration screening data in Gyrolab immunoassays allow selection of candidates from Set 1 (n = 230) for down-stream characterization.
From: Computationally restoring the potency of a clinical antibody against Omicron
a-d, Parental mAb COV2-2130 (orange circles) and positive control mAb S309 24 (magenta squares) serve as references for computationally designed mAbs in single-concentration immunoassays. Computationally designed antibodies are shown as gray diamonds; selected computationally designed antibodies are highlighted with other colors and symbols as shown in the legend. Target antigens are (a) wild type WA1/2020, (b) Delta, (c) Omicron BA.1 and (d) Omicron BA.1.1. Each screened antibody and antigen combination was evaluated in two replicate assays, shown as individual points in the plot. Each of the controls was replicated in two replicate assays for each of three groups of antibodies, resulting in six replicate assays for each point on the control curves. Control curve points and error bars indicate mean and standard deviation. Curves represent a four-parameter logistic curve fit to the control data. All analysis performed in GraphPad Prism.
