Fig. 1: The TWA1 locus.
From: The temperature sensor TWA1 is required for thermotolerance in Arabidopsis
a, The A. thaliana twa1-1 mutant is conspicuously thermosensitive. Five-day-old seedlings of WT and mutants with ABA-hypersensitive gene regulation were exposed to 45 °C for 90 or 105 min or not after 90 min priming at 38 °C. Pictures were taken 5 days after heat stress. b, The acquired thermotolerance of WT, twa1-1 and twa1-2 plants, as well as mutants complemented with the TWA1 gene, that were assayed for root growth within 5 days after heat exposure as indicated. c, ABA-inducible pHB6:LUC expression in 5-day-old seedlings exposed to 1 µM ABA for 16 h at 17 °C or 27 °C. Left, photographs and light emission of WT and twa1-1 plants expressing the reporter. Right, reporter activity per seedling given in relative light units (RLU; n = 18). d, Prediction of intrinsically disordered regions (https://metapredict.net/) with maximum score of 1, and a schematic of TWA1, showing the highly variable region (HVR, yellow) and two EAR domains (red) (top). TWA1 disruption in mutants is shown by arrowheads. Bottom, amino acid identity (red) of the HVR from A. thaliana (A.t.) and A. lyrata (A.l.). For b, data are mean ± s.d.; n = 6 per datapoint. Experimental and statistical significance details are provided in the Methods and as Source Data.
