Extended Data Fig. 10: Transcript levels in seedlings expressing TWA1 or TWA1 orthologs in comparison to wild type and twa1 mutants.
From: The temperature sensor TWA1 is required for thermotolerance in Arabidopsis
a, Left, Changes in transcript abundance for HSPs and ABA-induced RD29B in wild type of 5 days-old seedlings exposed to a shift from 22 °C to 37 °C for up to 6 h. Transcripts were quantified by realtime PCR after 4 h recovery at 22 °C. The transcript level in WT (pHB6:LUC) without heat treatment was set to 1. b, Transcript changes in TWA1-deficient and TWAoe lines compared to WT as in a, with 37 °C exposure for 4 h (treatment and symbols as in a. Transcripts were analysed by RT-PCR). c, Analysis as in b, however, with a recovery time of 0.5 h. d, Transcript changes of HSFA2, HSP21, and HSP26.5 affected by TWA1 and orthologs in 5-d old seedlings from ten different lines grown at 20 °C and exposed for 1 h to 25 °C, or kept at 20 °C, with 0.5 h recovery period at 20 °C prior to RT-qPCR analysis. e-g, Transcript abundance in A. thaliana seedlings grown at 20 °C and exposed to 35 °C for 1 h after 30 min recovery at 20 °C that expressed TWA1, the orthologues from A. lyrata AlTWA1 and Sinapis alba SaTWA1 under the control of the viral 35 S promoter in the twa1-2 background. The analysis of two representative lines is shown in comparison to WT lines (pHB6:LUC and Col-0) and both twa1 mutants for e, HSFA2, f, HSP21 and g, HSP26.5 transcript levels. The transcript abundance of WT pHB6:LUC line at 20 °C is set to 1. a-g, Data are mean ± s.d., n = 3, ten seedlings per n. Statistical significance in SI_Extended Fig. 10.
