Extended Data Fig. 1: Seedlings with enhanced ABA signalling are impaired in acquired thermotolerance.
From: The temperature sensor TWA1 is required for thermotolerance in Arabidopsis
a, Phenotypes of 5-day-old seedlings assayed for acquired tolerance. Pictures of wild type and lines with altered ABA response were taken one day after or without 150 min heat stress at 45 °C. Scale bar depicts 1 cm. b, Scheme of heat exposures in a. c, d, Inhibition of root growth in lines with reduced (blue) and increased ABA response (red) compared to WT (reporter line pHB6:LUC in Col-0 accession (d), or Col-0 (c)) exposed to 45 °C for different times. Inhibition was determined 5 days after stress. e, Inhibition of root growth by heat stress in the dark. 4 day-old acclimated seedlings of wild type, mutants pp2c_a and pp2c_b, and RCAR1 and RCAR6 overexpressing lines were exposed to 45 °C heat stress for the indicated times in darkness. Root extension was measured 5 days after treatment. c-e, Mean ± s.d. (n = 6). Statistical significance and source data are provided in the Supplementary Information (SI) File Extended Data Fig. 1.
