Extended Data Fig. 2: Hypersensitive ABA reporter response of twa1 mutants.
From: The temperature sensor TWA1 is required for thermotolerance in Arabidopsis
a, Induction of luciferase activity by exogenous ABA in wild type reporter line pHB6:LUC and twa1-1 mutant of pHB6:LUC genotype. 5-day-old seedlings were exposed to ABA for 24 h before luminescence measurements (mean ± SD, n = 36 per data point). b, ABA-induced luciferase in wild type pRD29B:LUC reporter line and twa1-1 mutant in the pRD29B:LUC genotype analysed as in a. c, Life imaging of pHB6:LUC or twa1-1 seedlings exposed to no and 30 µM ABA. Bright field and light emission photographs in false colours for relative light units (CCD-RLU) per seedling. Scale bar: 1 cm. d, Partial haplo-insufficiency of twa1-1 and twa1-2 alleles. F1 crosses and lines homozygous for the reporter were exposed to 3 µM ABA for 24 h (mean ± SD, n = 10). Statistical significance in SI_Extended Fig. 2.
