Extended Data Fig. 4: Yeast-Two-Hybrid analysis of TWA1-protein interaction.
From: The temperature sensor TWA1 is required for thermotolerance in Arabidopsis
Top, Physical interaction of TWA1 with JAM transcription factors of A. thaliana. TWA1 fused to the GAL4-activation domain (AD) was assayed for interaction with JAMs/MYC2 fused to Gal 4-DNA binding domain (BD) at 30 °C. Middle, transcriptional corepressors TPL and TPRs bind to TWA1 at 30 °C. Bottom, the interaction of TPL with the thermosensor is temperature dependent. Yeasts were grown in the absence of leucine and tryptophan (-L-W), and with or without histidine (H) using serial tenfold culture dilutions. Controls are empty expression cassettes (LV).
