Extended Data Fig. 5: GFP-TWA1 is functional in protoplasts and GFP-TWA1 abundance in response to temperature in yeast.
From: The temperature sensor TWA1 is required for thermotolerance in Arabidopsis
a, Capacity of TWA1 and GFP-TWA1 to inhibit ABA signalling in twa1-2 protoplasts. The ABA-responsive luciferase reporter (pRD29B:LUC construct) was induced by 10 µM exogenous ABA. Protoplasts were transfected with DNA of the effector expression cassettes (0 to 3 µg DNA/105 protoplasts) and incubated for 16 h at 25 °C before determination of the reporter activity (RLU) normalized to a cotransfected control (RFU). Data are mean ± SD, n = 3. b, GFP fluorescence in nuclei (n = 12) of yeast cells expressing GFP-TWA1 grown at 20 °C and 35 °C. The GFP signal was normalized to background fluorescence. Differences were statistically not significant (two-sided Mann-Whitney U Test, P = 0.30). Box plots: centre line, median; box limits, upper (25th percentile) and lower quartils (75th percentile); whiskers, 1.5 times interquartile range. Statistical significance in SI_Extended Fig. 5.
