Extended Data Fig. 6: S. arctica nuclear remodelling in response to membrane and microtubule inhibition.
From: Life-cycle-coupled evolution of mitosis in close relatives of animals
a, S. arctica cells arrest with cerulenin treatment. b, Maximum intensity projections of cerulenin-treated S. arctica interphase and mitotic nuclei immunolabelled for tubulin and NPCs. c, S. arctica spindles collapse with acute microtubule inhibition (0.5 μg/ml MBC for 15 min). Maximum intensity projections of representative nuclei of cells treated with DMSO and MBC and immunolabelled S. arctica spindles at different stages of mitosis. d-e, Maximum intensity projections of U-ExM images of S. arctica nuclei with tubulin (Tub - red) and pan-labelling (NHS) show loss of spindle MTs, while short astral MTs (d) and NPC radial arrays (blue) (e) still persist. f, Schematic representation of nuclear remodelling with increased polar indentation and spindle collapse in response to acute MT inhibitor treatment. Three independent experiments. Scale bars, 50 μm (a); 2 μm (b,d,e); 5 μm (c).
