Extended Data Fig. 7: Radial distribution of NPCs surrounding the S. arctica MTOC.
From: Life-cycle-coupled evolution of mitosis in close relatives of animals
a, Nuclear pore complexes (NPC- MAb414 antibody, blue) organised in radial arrays along MTs (tubulin, red). Images are representative maximum intensity projections of U-ExM stained S. arctica interphase nuclei from three biological replicates. b, 3D model of NPC arrays (dark blue) radiating from the MTOC (yellow) overlaid on an orthoslice of a representative S. arctica interphase nucleus imaged by FIB-SEM. c, NPCs are present at the centre of the MTOC in a subpopulation of nuclei. Representative maximum intensity projections of U-ExM images labelled with pan protein label NHS-ester (grey) and MAb414 antibody (NPC, blue). Yellow arrowhead indicates MTOC position. d, Cladogram of Ichthyosporea shows the position of Sphaeroforma sp. within the Ichthyophonida (teal) and neighbouring Dermocystids (orange). NPCs are organised in radial arrays across three distinct Sphaeroforma species. Images shown are representative maximum intensity projections of nuclei from Sphaeroforma sister species, S. nootkatensis, S. napiecek and S. gastrica are shown. e, NPCs are disorganised in S. arctica on prolonged microtubule inhibitor carbendazim (MBC) treatment (25 μg/ml MBC for 4 h). Images shown are maximum intensity projections of MBC-treated nuclei with pan protein labelling (NHS-ester) and MAb414 immunostaining. Yellow arrowhead indicates MTOC position. Scale bars, 5 μm (a,c,e); 500 nm (b); 2 μm (d).
