Extended Data Fig. 8: C. perkinsii shows features of human spindles.
From: Life-cycle-coupled evolution of mitosis in close relatives of animals
a, Schematic showing features of mitotic spindles including kinetochore and bridging fibres, interkinetochore distance (dkc), twist, spindle length and width. b-f, Plot of spindle length; P < 0.0001, nCperk = 28, nRPE1 = 10 (b), spindle width; P < 0.0001, nCperk = 28, nRPE1 = 10 (c), spindle width/length ratio; P = 0.00015, nCperk = 28, nRPE1 = 10 (d), interkinetochore distance; P < 0.0001, nCperk = 28, nRPE1 = 28 (e) and absolute value of spindle twist; P = 0.2742, nCperk = 16, nRPE1 = 34 (f) in C. perkinsii and human RPE1 cells. Data for RPE1 cells in (d) and (e) was taken from Štimac et al.27. Statistical analysis was performed using two-tailed Student’s t-test. Three independent experiments. Data are mean (black line) ± s.d (dark grey). The light grey area marks the 95% CI on the mean. g, Table summarizing spindle parameters (b-f) in C. perkinsii and human RPE1 cells. n is indicated in brackets.
