Fig. 3: C. perkinsii undergoes open mitosis.
From: Life-cycle-coupled evolution of mitosis in close relatives of animals
a, Representative images of cell cleavages in C. perkinsii cells at different life cycle stages labelled for: left, cell membrane with FM4-64; right, actin (purple) and DNA (grey). b, Representative maximum intensity projections of C. perkinsii nuclei at different stages of the cell cycle (I, P, M, A and T), in cells labelled for tubulin (red), DNA (grey) and NPCs (blue). c, C. perkinsii spindle in metaphase coloured for depth as shown in the legend on the left (top); enlarged section of the spindle showing kinetochore and bridging fibres (bottom) analysed in d and e and Extended Data Fig. 8. d, Bridging fibre thickness relative to the kinetochore fibre thickness (I, intensity); P < 0.0001. e, Interkinetochore distance (d) scaled to the spindle long axis; P = 0.1512. Data for RPE1 cells in d and e were taken from ref. 27 nCperk = 28, nRPE1 = 10 spindles. Spindles were analysed over three independent experiments. We performed statistical analysis using two-tailed Student’s t-test. Data are mean ± s.d. f, Single slice from a representative electron tomogram of C. perkinsii mitotic nucleus overlaid with a 3D model showing the spindle microtubles (red), chromosomes (green) and NE (blue). The purple arrowhead indicates the centriole in focus. Scale bars, 5 μm (a,b), 500 nm (f). P, prophase.
