Extended Data Fig. 3: Duplication and NE embedding of the S. arctica MTOC. | Nature

Extended Data Fig. 3: Duplication and NE embedding of the S. arctica MTOC.

From: Life-cycle-coupled evolution of mitosis in close relatives of animals

Extended Data Fig. 3

a, Cartoon of S. arctica MTOC cycle, showing nuclei with 1 or 2 MTOCs. b, Dot plot showing proportion of cells with 2 MTOCs in control and hydroxyurea (HU) treated cells. The dots are shaded to indicate the three biological replicates (n16h_ctrl = 121, 166 & 157, n16h_HU = 108, 155 & 168, n24h_ctrl = 111, 152 & 193). Statistical analysis was performed using a two-tailed Mann–Whitney U-test; P = 0.1000, not significant. Data are mean ± s.d. c, Dot plot showing dimensions of S. arctica MTOCs for nuclei in interphase and mitosis. Width and height of MTOCs were measured from NHS-ester labelled U-ExM gels (ninterphase = 29, nmitosis = 60 MTOCs) Statistical analysis was performed using a two-tailed Mann–Whitney U-test; Width-Interphase vs Mitosis P  =  0.1021; Height-Interphase vs Mitosis P < 0.0001. Nuclei analysed over three biological replicates. Data are mean ± s.d. (ns-not significant; ****P < 0.0001). c, Single slices through representative FIB-SEM volumes showing different configurations of the S. arctica MTOC-NE interface, from outer nuclear membrane (ONM) association in interphase to inner nuclear membrane (INM) embedding in mitosis. Blue asterisks indicate nuclear pore complexes (NPCs). Scale bars, 300 nm (d).

Source Data

Back to article page