Extended Data Fig. 3: Duplication and NE embedding of the S. arctica MTOC.
From: Life-cycle-coupled evolution of mitosis in close relatives of animals

a, Cartoon of S. arctica MTOC cycle, showing nuclei with 1 or 2 MTOCs. b, Dot plot showing proportion of cells with 2 MTOCs in control and hydroxyurea (HU) treated cells. The dots are shaded to indicate the three biological replicates (n16h_ctrl = 121, 166 & 157, n16h_HU = 108, 155 & 168, n24h_ctrl = 111, 152 & 193). Statistical analysis was performed using a two-tailed Mann–Whitney U-test; P = 0.1000, not significant. Data are mean ± s.d. c, Dot plot showing dimensions of S. arctica MTOCs for nuclei in interphase and mitosis. Width and height of MTOCs were measured from NHS-ester labelled U-ExM gels (ninterphase = 29, nmitosis = 60 MTOCs) Statistical analysis was performed using a two-tailed Mann–Whitney U-test; Width-Interphase vs Mitosis P = 0.1021; Height-Interphase vs Mitosis P < 0.0001. Nuclei analysed over three biological replicates. Data are mean ± s.d. (ns-not significant; ****P < 0.0001). c, Single slices through representative FIB-SEM volumes showing different configurations of the S. arctica MTOC-NE interface, from outer nuclear membrane (ONM) association in interphase to inner nuclear membrane (INM) embedding in mitosis. Blue asterisks indicate nuclear pore complexes (NPCs). Scale bars, 300 nm (d).