Extended Data Fig. 6: Histological and functional validation of dopamine transients evoked after µORs KD in VTA.
From: Distinct µ-opioid ensembles trigger positive and negative fentanyl reinforcement
(A) Localization of the NAc recording site in VTA µORs KD (n = 8 mice) and CTL (n = 7 mice) animals plus viral infection in VTA. Bottom: (B) Representative histological example of fibre implantation in NAc and virus infection in VTA. (C) Representative example of NAc raw dLight signal (470 and 405 nm) in control (Top) and VTA µORs KD (Bottom) animals after i.p. injection of saline, Fentanyl (0.3 mg/kg) and apomorphine (10 mg/kg). (D) Accumbal dLight signal (ΔF/F0) in VTA µORs KD (red, n = 8 mice) vs CTL mice (grey, n = 7 mice) after i.p. injection of apomorphine at 10 mg/kg. (E) Mean ± SEM of the area under the curve after pharmacological i.p. injection of apomorphine in VTA µORs KD (n = 8 mice) and CTL (n = 7 mice). (F) Normalization of AUC (Mean ± SEM) evoked by fentanyl 0.3 mg/kg i.p. injection compared to the AUC of the partial agonist D1/D2 dLight apomorphine in VTA µORs KD (n = 8 mice) and CTL (n = 7 mice) (Mann-Whitney test, two-sided, p > 0.05).
