Extended Data Fig. 7: Behavioral effects during oGABAsi-fentanyl occlusion and locomotion reduction after VTA µOR-KD.
From: Distinct µ-opioid ensembles trigger positive and negative fentanyl reinforcement
(A) i.p. protocol fentanyl injection. (B) Average speed locomotion induced by fentanyl at different doses in 1 h open field test (n = 7 mice). (C) Correlation matrix of the different behavioral parameters during oGABAsi occlusion. Superimposed are annotated the Spearman correlation coefficient (n = 7 mice). (D) Active lever press number (ALP, Mean ± SEM) through the session of oGABAsi occlusion after different dose of fentanyl i.p. injected (n = 7 mice). (E) Frequency distribution of latency between ALP during oGABAsi occlusion experiment after different dose of fentanyl i.p. injected (n = 7 mice). (F) Schematic of the mice preparation to induce VTA µORs deletion. (G) Distance travelled (Mean ± SEM) after i.p. injection of saline (sal) or Fentanyl 0.2 mg/kg in mice with VTA µORs KD (n = 4 mice, red), control animal (n = 4 mice, grey) or control saline animal (n = 4 mice, black).
