Extended Data Fig. 8: VTA µOR-KD prevents conditioned place preference.
From: Distinct µ-opioid ensembles trigger positive and negative fentanyl reinforcement
(A) Schedule of the experiment to induce Place preference with i.p. injection of fentanyl 0.3 mg/kg. (B) Representative example of occupancy plot for control mice (Left) and VTA µORs KD mice (Right), before (top) and after (Bottom) conditioning. (C) Left: Average preference score (Mean ± SEM) for the fentanyl compartment before and after conditioning in CTL mice (Black, n = 11) and VTA µORs KD mice (red, n = 10) (Two way RM ANOVA, Session, F(1,38) = 10.91, p < 0.01, Genotype, F(1,38) = 0.8352, p > 0.05, Session x Genotype, F(16,38) = 2.046, p > 0.05; Bonferroni post hoc analysis, **p = 0.029). Right: Average difference of time (in seconds, Mean ± SEM) spent in the fentanyl compartment for CTL mice vs VTA µOR KD mice (Right, Mann-whitney test, two-sided, p > 0.05).
