Extended Data Fig. 9: Real-time place aversion (RTPA) induced by CeA µOR-neurons activation.
From: Distinct µ-opioid ensembles trigger positive and negative fentanyl reinforcement
(A) Schematic of the mice preparation for optogenetically manipulating CeA µORs-expressing neurons during a real-time place aversion task. (B) Schedule of the experiment to induce real-time place aversion with 20 Hz stimulation, consisting of a pre-session (20 min), three sessions of conditioning (30 min) and a post-conditioning session (20 min) (C) Relative time spent in the stimulating chamber (Mean ± SEM) during the behavioral task (n = 9 for ChR2 group and n = 15 for the GFP group). (D) Average (Mean ± SEM) relative time spent in the conditioning chamber over the 3 conditioning days (n = 9 for ChR2 group and n = 15 for the GFP group; Unpaired t test, two-sided, *p = 0.028).
