Fig. 2: Cellular and anatomical characterization of µOR-expressing CeA neurons.
From: Distinct µ-opioid ensembles trigger positive and negative fentanyl reinforcement
a, Left, representative example of CeA imaged by single-molecule fluorescent in situ hybridization (smFISH) for Oprm1 (blue), somatostatin (Sst) (green) and Prkcd (red) mRNA. Right, quantification of co-localization between Sst- and Prkcd-positive neurons (n = 7 mice). Scale bar, 100 μm. b, Left, representative example of CeA imaged by smFISH for µOR (Oprm1) (white), Sst (green) and Prkcd (red). Right, quantification of Oprm1-positive neurons among the Sst+ and Prkcd+ populations. Data are mean ± s.e.m. (n = 7 mice). Two-sided Mann–Whitney test, ***P = 0.0006. Scale bar, 20 μm. c, Schematic of mouse preparation to visualize the µOR-expressing CeA efferent pathway (top) and representative image showing injection (bottom). Scale bar, 100 µm. d, Projection site of µOR-expressing CeA neurons in the parabrachial lateral nucleus (PBL) and the ventral bed nucleus of the stria terminalis (BNSTv) and dorsal bed nucleus of the stria terminalis (BNSTd). Scale bars, 1 mm.
