Extended Data Fig. 1: Behavioral quantification of fentanyl precipitated withdrawal.
From: Distinct µ-opioid ensembles trigger positive and negative fentanyl reinforcement
(A) Representative example of occupancy (left) and distance traveled (Right) after i.p. injection of fentanyl (Reward, left panel) or during precipitation of withdrawal by an i.p. injection of naloxone (Withdrawal, right panel). (B) Box Plot representation (Quartils and median, whiskers min to max) of the seven withdrawal symptoms quantified in dependent animal with no precipitation (grey, n = 14), in dependent animal with precipitation (red, n = 13) and in non-dependent animal with naloxone injection (white, n = 13). (During fentanyl: Kruskal-Wallis test: Rearing, H(3) = 31.72, P < 0.001, Jump, H(3) = 2.077, Defecation, H(3) = 26.32, P < 0.001, Wet Dog Shake, H(3) = 33.37, P < 0.001, Body Licking, H(3) = 37.14, P < 0.001, Immobility Time, H(3) = 20.47, P < 0.001, Distance, H(3) = 25.84, P < 0.001; During Naloxone: Kruskal-Wallis test: Rearing, H(3) = 27.67, Jump, H(3) = 26.79, P < 0.001, Defecation, H(3) = 21.65, P < 0.001, Wet Dog Shake, H(3) = 25.83, P < 0.001, Body Licking, H(3) = 30.14, P < 0.001, Immobility Time, H(3) = 28.94, P < 0.001, Distance, H(3) = 26.70; P < 0.001; Dunn’s multiple comparisons test, *p < 0.05, **p < 0.01, ***p < 0.001). (C) Heat-map representation of the different precipitated withdrawal symptoms normalized individually from min to max (respectively 0 and 1). (D) Correlation matrix of the individual precipitated withdrawal symptoms. Superimposed are annotated the Spearman correlation coefficient.
