Extended Data Fig. 2: Anatomical validation of the KD experiment.
From: Distinct µ-opioid ensembles trigger positive and negative fentanyl reinforcement
(A) Schematic of the mice preparation to induce VTA µORs deletion. (B) Fluorescent in situ hybridization (RNAscope) for Oprm1 (µORs, red) and DAPI (blue) in VTA (Left) or CeA (Right) without (Top) or with µORs deletion (Bottom) (C) Quantification of mRNA puncta (Mean ± SEM) per images in control (black) vs KD animals (red) respectively in VTA (Left) and CeA (Right). For VTA, n (images) = 14 and n (images) = 10, respectively for KD animals (n = 3 mice) and control animals (n = 3 mice). For CeA, n (images) = 16 and n (images) = 16, (n = 7 mice) (unilateral injection). Mann-Whitney test, two-sided, ***p < 0.001.
