Extended Data Fig. 3: Unbiased behavioral validation of µORs deletion after fentanyl and naloxone treatments.
From: Distinct µ-opioid ensembles trigger positive and negative fentanyl reinforcement
(A) Top: Representative example of the injection site in the different brain regions. Bottom: Localization of the injection centre in the different mice (CTL (n = 12); VTA (n = 13); NAc (n = 13); BLA (n = 12); CeA (n = 14); PVT (n = 13)). (B) Names of the variable extracted for DLC tracking. (C-D) Top: Spider plot representing the multidimensional distance between each experimental groups (µORs KD in different brain regions) vs control group (non-KD) of each time-series quantified after precipitation of withdrawal. Bottom: SVM classification of the 15 variables extracted from DLC in the different experimental groups (µORs KD) vs control group after precipitation of withdrawal. Color code used to evaluate the accuracy of prediction of each variables extract. (estimated p value with permutation test; *p < 0.05, **p < 0.01, ***p < 0.001).
