Extended Data Fig. 4: Anatomical validation of VTA µORs expressing neurons.
From: Distinct µ-opioid ensembles trigger positive and negative fentanyl reinforcement
(A) Left: Low VTA magnification representative example of fluorescent in situ hybridization for opioid receptor mu 1 (Oprm1, Blue), solute carrier family 32 member 1 (Slc32a1, green) and solute carrier family 6 member 3 (Slc6a3, red) mRNAs (Scale bars: 100 µm). Right: Quantification of colocalization between Slc32a1 (DAT) and Slc6a3 (GABA) positive neurons (n = 5 mice). (B) Left: Fluorescent in situ hybridization representative example of VTA molecular marker for Oprm1 (µORs, White), Slc32a1 (GABA, green) and Slc6a3 (DA, red). Right: Proportion of GABA and DA neurons (Mean ± SEM) within the µORs-expressing neurons (n = 5 mice). Mann-Whitney test, two-sided, **p = 0.0079.
