Extended Data Fig. 5: Example traces of evoked DA and GABA activity after pharmacological i.p. injection.
From: Distinct µ-opioid ensembles trigger positive and negative fentanyl reinforcement
(A) Localization of the recording site in DAT-cre (n = 8) and GAD-cre (n = 8) animals. (B) Representative histological example of virus infection and fibre implantation in VTA. (C,F) Raw signal recording example (470 and 405 nm) of GCaMP6m after i.p. injection of Naloxone in naïve mice (Left), after i.p. injection of fentanyl (middle) and after fentanyl plus naloxone i.p. injection (right) in dopaminergic neurons (Pink) and GABAergic neurons (Green). (D,G) Example Ca2+ signal (ΔF/F0) of dopamine (Pink) and GABA (green) VTA neurons and its associated transients identification (Blue). (E,F) Average transients rate (Mean ± SEM) after i.p. injection of Naloxone in naïve mice, after i.p. injection of fentanyl and after fentanyl plus naloxone i.p. injection in dopaminergic neurons (pink) and GABAergic neurons (green) (For DAT transients, Kruskal-Wallis test H(7) = 21.66, P < 0.01, For GAD transients, Kruskal-Wallis test H(7) = 6.788, P > 0.05, Dunn’s multiple comparisons test, *p < 0.01).
