Extended Data Fig. 6: snRNAseq confirms cholangiocyte diversity and ductal reaction in late-stage disease.
From: Acquisition of epithelial plasticity in human chronic liver disease
a-b) Cholangiocyte UMAP with overlaid gradient of expression of large cholangiocyte markers MUC5B and MUC1 c-d) Cholangiocyte UMAP with overlaid gradient of expression cholangiocyte marker CFTR and small cholangiocyte marker BCL2. e) UMAP indicating disease stage of cells. f-g) Cholangiocyte UMAP with overlaid gradient of expression of ductal reaction markers TNFRS12A and NCAM1. h-i) Quantification of the proportion of cholangiocytes expressing ductal reaction markers TNFRS12A and NCAM1 across disease stages. p-values indicated (two-sided Fisher exact test). j) Immunostaining of K7 and NCAM1 in end stage tissue sections. Scale bar = 15 um.
