Extended Data Fig. 7: Characterisation of biphenotypic cells suggests an absence of an adult stem or foetal progenitor population.
From: Acquisition of epithelial plasticity in human chronic liver disease
a) Heatmap of relative expression of large cholangiocyte markers MUC1 and MUC5b and small cholangiocyte marker BCL2 across the indicated cell types. b) Cluster 9 cholangiocytes identified in Fig. 3d plotted as a proportion of cholangiocytes from each disease stage. c) Cluster 5 cholangiocytes identified in Fig. 3d plotted as a proportion of cholangiocytes from each disease stage. d) Cluster 1 cholangiocytes identified in Fig. 3d plotted as a proportion of cholangiocytes from each disease stage. P-values indicated. (Binomial Generalized Linear Mixed- Effects Model (BOBYQA optimiser, maxfun = 2e5) with patient ID as a random effect). e) Violin plots of expression of indicated hepatocyte and cholangiocyte markers comparing the hepatocyte, cholangiocyte and biphenotypic populations. f) Upset plot displaying the number of biphenotypic hepatocytes co-expressing the indicated stem/ progenitor cell genes. g-i) End stage hepatocyte and cholangiocyte UMAP with overlaid gradient of expression for indicated stem cell markers. j) Heatmap of relative expression of senescence markers CDKN2A and CDKN1A in bi- phenotypic hepatocytes across disease progression. k-i) End stage hepatocyte and cholangiocyte UMAP with overlaid gradient of expression for indicated liver progenitor cell markers.
