Extended Data Fig. 8: Plasticity markers are expressed in end stage liver.
From: Acquisition of epithelial plasticity in human chronic liver disease
a) Immunofluorescence staining for HepPar1, K7 and SOX4 (upper panel) K23, K7 and SOX4 (lower panel) in end stage tissue sections. Scale bars = 15 um (lower panel) and 10um (upper panel). b) Immunofluorescence staining for HepPar1 and K23 in end stage tissue sections. Scale bar = 15 um. c) Immunofluorescence staining for ALB, K19 and NCAM1 in healthy and end stage tissue sections. Yellow box indicates the region shown in higher magnification. Scale bars = 50 um upper 2 panels and 10 um lower 2 panels. d) Immunofluorescence staining for HepPar1 and K23 in end stage tissue sections. Scale bar = 20 um. e) Immunofluorescence staining for ALB, K19 and KLF6 in healthy and end stage tissue sections. Scale bars = 30 um upper 2 panels and 10 um lower panel. n = 3 healthy and 3 end stage patient tissue samples (a-e). f) Upset plot displaying the number of biphenotypic hepatocytes co-expressing the indicated plasticity and proliferative genes.
