Extended Data Fig. 10: Extracellular matrix composition alters ICOs branching and differentiation.
From: Acquisition of epithelial plasticity in human chronic liver disease
a-d) qPCR for hepatocyte and cholangiocyte marker expression in dICOs treated with wither vehicle or indicated treatment for the duration of the differentiation. n = 3 biologically independent experiments, P-values are indicated unpaired t-test. Errors bars indicate mean with SD. e) Heatmap showing relative expression of YAP signalling genes across disease stages in hepatocytes and cholangiocytes combined. f) Examples of dICOs differentiated in a mixture of 50:50 collagen I: Matrigel (lower 2 panels). Immunofluorescence staining for ALB and K19. White box indicates region shown in high magnification (lower panel). uICOs grown in 100% Matrigel included as a control (upper panel). n = 3 patient organoid lines. Scale bars = 100 um upper, 200 um middle and 150 um lower panel.
