Extended Data Fig. 1: Biphenotypic cells are observed in late stages of the disease progression.
From: Acquisition of epithelial plasticity in human chronic liver disease
a) Immunofluorescent staining for ALB, K19 and K7 on tissue sections from healthy, MASLD, MASH and Cirrhosis staging. Scale bars = 50 um for Healthy MASLD and MASH panels and 20um for cirrhosis panels b) H&E and collagen staining of healthy and end stage MASLD tissue sections. Scale bars = 500 um Healthy H&E, 1000 um End stage H&E, 2000 um for Healthy and End stage collagen staining. c) Staining for KRT7 and HepPar1 in end stage liver. An example of a double positive cell is indicated (yellow arrow). Scale bars = 20 um. d) Immunohistochemistry staining for K19 on tissue section of the indicated disease stage. In end stage (bottom panels), a cell with hepatocyte morphology expressing low levels of K19 is indicated in the left panel (yellow arrow) and a cell negative for K19 within a duct with hepatocyte morphology is indicated in the right panel (yellow arrow). Scale bars = 50 um (4 upper panels) and 20 um (4 lower panels). n = 3 patient samples from each indicated disease stage in (a-d).
