Fig. 1: MYCT1 is crucial for human HSPC ex vivo expansion and engraftment.
From: MYCT1 controls environmental sensing in human haematopoietic stem cells
a, MYCT1 expression from RNA-seq on sorted populations from human 5–6-week AGM, placenta, yolk sac (YS), second trimester FL, CB and adult bone marrow (ABM). n = 6 (AGM), n = 4 (placenta) or n = 3 (YS, FL, CB, ABM) donors per tissue, mean ± s.e.m. FPKM, fragments per kilobase of mappable length and million counts. CMP, common myeloid progenitor; MEP, megakaryocyte erythroid progenitor; GMP, granulocyte macrophage progenitor; CDP, common dendritic cell progenitor; CLP, common lymphoid progenitor. b, MYCT1 expression in human FL GPI80+ and GPI80– HSPC (CD34+CD38–CD90+) subsets30, and UM171-expanded CB ITGA3+ and ITGA3– HSPC (CD90+EPCR+CD133+CD34+CD45RA–) subsets19. n = 3 and 4 replicates, mean ± s.e.m., two-tailed paired and unpaired t-test, respectively. c, Relative MYCT1 expression by qPCR with reverse transcription (RT–qPCR) in CB HSPCs (CD34+CD38–CD90+) sorted before and after culture. n = 3 experiments, mean ± s.e.m., two-tailed paired t-test. d, Fold ex vivo expansion of all cells, total HSPCs (CD34+CD38–CD90+CD45RA–) and immunophenotypic long-term HSCs (LT-HSCs; CD34+CD38–CD90+CD45RA–EPCR+ITGA3+) from control or MYCT1 KD CB. n = 8 (control), n = 12 (KD1), n = 5 (KD2) replicates from 4 independent experiments, mean ± s.e.m., two-tailed Mann–Whitney test. Left to right: all cells KD1: P = 0.041, P = 0.000016, P = 0.000059, P = 0.000037; KD2: P = 0.0016, P = 0.0016, P = 0.0007, P = 0.0007; total HSPC KD1: P = 0.0055, P = 0.0022, P = 0.0044, P = 0.0004; KD2: P = 0.0062, P = 0.0031, P = 0.0027, P = 0.0027; LT-HSC KD1: P = 0.0252, P = 0.0002, P = 0.000022, P = 0.0008; LT-HSC KD2: P = 0.0186, P = 0.0016, P = 0.0007, P = 0.0083. e, Quantification of human haematopoietic engraftment (human CD45+) in NSG mice transplanted with equal numbers (5,000) of sorted control or MYCT1 KD CB HSPCs. n = 6 (control) and n = 5 (MYCT1 KD) mice per group, mean ± s.e.m., two-tailed Mann–Whitney test. Left to right: P = 0.0303, P = 0.0043, P = 0.0087, P = 0.0173, P = 0.0303.
