Extended Data Fig. 5: Analysis of MYCT1 associated programs in independent human and mouse datasets reflecting different levels of HSC competence.
From: MYCT1 controls environmental sensing in human haematopoietic stem cells
a,b Evaluating the expression of MYCT1 associated “stemness” programmes in uncultured compared to 3 day cultured CB LT-HSC (CD34+CD38−CD90+CD45RA−CD49f+)28. Heatmaps depict Log Fold change. c-e Evaluating the expression of Myct1 and MYCT1 associated human HSPC “stemness” programmes in mouse repopulating and non-repopulating HSC and progenitors in PVA-cultured mouse HSPCs41. Mouse sorted immunophenotypic HSPCs (ELSK: EPCR+Lin−Sca-1+C-kit−) which were able repopulate mice upon transplantation (ELSK_rep) were compared to non-repopulating ELSK cells (ELSK_non rep), as well as both repopulating and non-repopulating non-ELSK progenitor cells (Prog)41. Heatmaps depict normalized gene expression.
