Extended Data Fig. 8: Multilineage differentiation after transplanting day 15 progeny of HSPCs with restored MYCT1 expression.
From: MYCT1 controls environmental sensing in human haematopoietic stem cells
a Table indicating the number of mice transplanted with the progeny of control or MYCT1 OE HSPC after 15 days in culture for each condition and replicate. b,c Gating strategy and representative FACS plots showing the human myeloid (CD14+ or CD66b+), B-lymphoid (CD19+), T-lymphoid/other (CD3, CD4 or CD8+), undifferentiated (CD34+CD38−), HPC (CD38+), megakaryocytes (CD41a+), and erythroid (GlyA+CD71+) populations of mice transplanted with the progeny of 500, 2500 (b) or 10000 (high dose) (c) HSPCs after 15 days in culture. d-f Percentage of total engraftment (hCD45+), GFP, and differentiated populations for mice transplanted with the progeny of 10000 HSPCs. n = 5 (control) or n = 6 (MYCT1 OE) mice. Percentage of cells in each population within the total BM hematopoietic compartment (human and mouse CD45) is indicated in the FACS plots. Median and all individual values are shown.
